She studied at the Stella Adler Studio of Acting Conservatory program and at the Susan Batson Studio with the renowned acting coach herself, both in New York. Suzie is an Austrian-Canadian actress, artist and punk rock singer. This encounter turns the two into accomplices in the confrontation with the volatility of existence. Their initially bumpy conversation gradually develops into a mutual deep understanding of the importance of accepting oneself independently of social norms, judgments and conventions. But their cultural differences give them the space to change their perspective on their personal realities. Two strangers from different cultures meet in absurd circumstances – a Karaoke Bar in Vienna-, as outcasts of society. Being able to bring your child to such a safe place, where they are well looked after, is an important socio-political and women’s rights achievement that is just as important today as it was 20 years ago. The first baby hatch was launched in Vienna in 2001 to help parents in such hopeless situations. When the external circumstances are precarious, a lot of feelings of guilt come up when having a child. ASTRONAUTS deals with questions that are drastic and still hard to make for women today. Discrimination, misunderstandings and fears are mostly the result of this. 2019, 25-65.The human brain automatically organizes people and things into categories to make the world easier to understand for themselves. Interferometric Scattering (iSCAT) Microscopy and Related Techniques. Physical Chemistry Chemical Physics 2020, 22 Phase separation in pore-spanning membranes induced by differences in surface adhesion. Tewaag, Thomas Vagedes, Ingo Mey, Claudia Steinem. Differential Diffusional Properties in Loose and Tight Docking Prior to Membrane Fusion. Agata Witkowska, Susann Spindler, Reza Gholami Mahmoodabadi, Vahid Sandoghdar, Reinhard Jahn.In vitro single vesicle fusion assays based on pore-spanning membranes: merits and drawbacks. Peter Mühlenbrock, Merve Sari, Claudia Steinem.The Journal of Physical Chemistry C 2019, 123 Nonfluorescent Optical Probing of Single Molecules and Nanoparticles. Interferometric Scattering Microscopy: Seeing Single Nanoparticles and Molecules via Rayleigh Scattering. Evaluation of Lateral Diffusion of Lipids in Continuous Membranes between Freestanding and Supported Areas by Fluorescence Recovery after Photobleaching. Azusa Oshima, Hiroshi Nakashima, Koji Sumitomo.Ultrahigh-Speed Imaging of Rotational Diffusion on a Lipid Bilayer. Mahdi Mazaheri, Jens Ehrig, Alexey Shkarin, Vasily Zaburdaev, Vahid Sandoghdar.ACS Applied Materials & Interfaces 2021, 13 Pore-Spanning Plasma Membranes Derived from Giant Plasma Membrane Vesicles. Baumann, Lena Strieker, Ulla Unkelbach, Claudia Steinem. Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins. This article is cited by 10 publications. We provide a detailed characterization of the diffusion behavior in these membrane systems and discuss future directions. Furthermore, we find clear indications for a decrease of the diffusion constant in the freestanding regions away from the pore center. We attribute this reduced diffusion on the supported parts in the case of oxygen-plasma-treated surfaces to larger membrane–substrate interactions, which lead to a higher membrane tension in the freestanding membrane parts. In the case of functionalization with gold and 6-mercapto-1-hexanol, similar diffusion coefficients are obtained for both the suspended and the supported regions, whereas for oxygen-plasma-treated surfaces, diffusion is almost 4 times slower in the supported parts of the membranes.
For both substrate treatments, diffusion in regions suspended on pores with diameters of 5 μm is found to be free. We study the location-dependent diffusion of DOPE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) lipids (DOPE) labeled with gold nanoparticles in (1,2-dioleoyl-sn-glycero-3-phosphocholine) (DOPC) bilayers prepared on holey silicon nitride substrates that were either (i) oxygen-plasma-treated or (ii) functionalized with gold and 6-mercapto-1-hexanol. Here, we resolve this issue by employing interferometric scattering microscopy (iSCAT). Although some properties of the suspended regions in PSMs have been characterized using fluorescence studies, it has not been possible to examine the mobility of membrane components on the supported membrane parts. We measure and compare lipid diffusion in the supported and suspended regions of PSMs prepared on a microfabricated porous substrate. Pore-spanning membranes (PSMs) provide a highly attractive model system for investigating fundamental processes in lipid bilayers.